Ic stress-induced genes data were obtained from this study at 24 hpt. doi:10.1371/journal.pone.0125666.tresponses. Expression profiling of plant response to one type of stress-B. cinerea infection or abiotic stress treatment- has been well-documented [21, 25, 35, 36]. In addition, transcriptome analysis of Arabidopsis, rice, tobacco (Nicotiana tabacum) and cotton (Gossypium hirsutum L.) revealed crosstalk of responsive genes to various abiotic stresses [37?0]. Combinations of different biotic and abiotic stresses have allowed the SP600125 site identification of candidate genes involved in broad resistance [41]. A recent transcriptome analysis showed shared regulated genes when Arabidopsis plants were infected with B. cinerea or treated with cold, drought or oxidative stress [20]. Here, we extended the comparative microarray analysis, obtained from Arabidopsis public databases, to include B. cinerea, heat, salinity and osmotic stresses. We identified upand down-regulated genes after treatments with an individual stress, or upon a combination of biotic and abiotic stresses. In response to B. cinerea, approximately 7 of genes were induced and 5 were repressed across the whole Arabidopsis transcriptome [20]. The transcript levels of 153 and 799 genes increased more than twofold after heat and high salinity treatments, respectively, compared with the control genes; but 507 and 872 genes had impaired transcript levels of the transcripts for the same treatments (Fig 1). The largest number of genes up- or down-regulated by a specific stress corresponded to osmotic stress with 1695 or 2210 genes, respectively. Previously, it was also found that the number of genes induced by salt stress in cotton was greater than in any other type of abiotic stress, particularly cold, pH or osmotic stress [40]. Based on the molecular and Grazoprevir biological activity functional classifications and comparisons, some abioticPLOS ONE | DOI:10.1371/journal.pone.0125666 May 1,15 /Microarray Analysis of Arabidopsis-Stressed Plantsstress-regulated genes have been classified as genes, with known functions such as transcription regulators, scavengersor ion transporters [39, 40]; yet many remain unknown. We closely looked to the relationship between gene regulation in response to B. cinerea infection and in response to heat, salinity or osmotic stresses. We found that osmotic stress and B. cinerea shared the highest number of regulated genes; while heat and B. cinerea shared the least. Although a significant number of differentially expressed genes were regulated under specific stresses; others were also co-regulated by a combination of different stresses. We observed strong correlations of stress-associated genes and found that 13 stress-inducible genes and 29 stressrepressible genes have responded to all four types of stresses (Fig 2). We expanded the analysis to include other transcriptome studies and we noticed that there were large fluctuations in the percentage of co-regulated genes (up- or down-regulated) between biotic (B. cinerea), and abiotic stresses, as shown in Table 1 as 58 cold, 12.9 drought, 17.2 oxidative stress, 10.1 heat, 37.9 salinity, and 89 osmotic stress (Table 1). Microarray transcriptional profiling demonstrated that lecithin:cholesterol acyltransferase 3 (LCAT3) gene, encoding for phospholipase A1 (PLA1) enzyme [42], was upregulated after infection with B. cinerea or treatment with heat, 150 mM NaCl or 300 mM mannitol (Fig 3). In addition, the expression of Arabidopsis LCAT3 in yea.Ic stress-induced genes data were obtained from this study at 24 hpt. doi:10.1371/journal.pone.0125666.tresponses. Expression profiling of plant response to one type of stress-B. cinerea infection or abiotic stress treatment- has been well-documented [21, 25, 35, 36]. In addition, transcriptome analysis of Arabidopsis, rice, tobacco (Nicotiana tabacum) and cotton (Gossypium hirsutum L.) revealed crosstalk of responsive genes to various abiotic stresses [37?0]. Combinations of different biotic and abiotic stresses have allowed the identification of candidate genes involved in broad resistance [41]. A recent transcriptome analysis showed shared regulated genes when Arabidopsis plants were infected with B. cinerea or treated with cold, drought or oxidative stress [20]. Here, we extended the comparative microarray analysis, obtained from Arabidopsis public databases, to include B. cinerea, heat, salinity and osmotic stresses. We identified upand down-regulated genes after treatments with an individual stress, or upon a combination of biotic and abiotic stresses. In response to B. cinerea, approximately 7 of genes were induced and 5 were repressed across the whole Arabidopsis transcriptome [20]. The transcript levels of 153 and 799 genes increased more than twofold after heat and high salinity treatments, respectively, compared with the control genes; but 507 and 872 genes had impaired transcript levels of the transcripts for the same treatments (Fig 1). The largest number of genes up- or down-regulated by a specific stress corresponded to osmotic stress with 1695 or 2210 genes, respectively. Previously, it was also found that the number of genes induced by salt stress in cotton was greater than in any other type of abiotic stress, particularly cold, pH or osmotic stress [40]. Based on the molecular and functional classifications and comparisons, some abioticPLOS ONE | DOI:10.1371/journal.pone.0125666 May 1,15 /Microarray Analysis of Arabidopsis-Stressed Plantsstress-regulated genes have been classified as genes, with known functions such as transcription regulators, scavengersor ion transporters [39, 40]; yet many remain unknown. We closely looked to the relationship between gene regulation in response to B. cinerea infection and in response to heat, salinity or osmotic stresses. We found that osmotic stress and B. cinerea shared the highest number of regulated genes; while heat and B. cinerea shared the least. Although a significant number of differentially expressed genes were regulated under specific stresses; others were also co-regulated by a combination of different stresses. We observed strong correlations of stress-associated genes and found that 13 stress-inducible genes and 29 stressrepressible genes have responded to all four types of stresses (Fig 2). We expanded the analysis to include other transcriptome studies and we noticed that there were large fluctuations in the percentage of co-regulated genes (up- or down-regulated) between biotic (B. cinerea), and abiotic stresses, as shown in Table 1 as 58 cold, 12.9 drought, 17.2 oxidative stress, 10.1 heat, 37.9 salinity, and 89 osmotic stress (Table 1). Microarray transcriptional profiling demonstrated that lecithin:cholesterol acyltransferase 3 (LCAT3) gene, encoding for phospholipase A1 (PLA1) enzyme [42], was upregulated after infection with B. cinerea or treatment with heat, 150 mM NaCl or 300 mM mannitol (Fig 3). In addition, the expression of Arabidopsis LCAT3 in yea.