Medium of the adipocyte cell line 3T3-L1 following direct challenge
Medium of the adipocyte cell line 3T3-L1 following direct challenge with LTA/PGN (3 g/ml; 100 g/ml) for 6 hours. Results The older age group, compared with the young group receiving the same stimulus, showed significantly elevated alveolar levels of MIF and CXCL1 (KC), both involved in neutrophil recruitment. Neutrophils (controls: 0.029 ?0.033, young: 13.9 ?7.8, old: 29.3 ?10.5, x 106) and the total number of cells (controls: 0.72 ?0.26, young: 16.4 ?9.7, old: 32.9 ?11.2, x 106) within the alveolar space were significantly and age-dependently increased, following pulmonary insult. Plasma adiponectin did not change significantly. However, instillation of LTA/PGN significantly elevated the levels of adiponectin found within the alveolar space (controls: 3.9 ?2.4, young: 22.5 ?8.8, old: 29 ?10.8 ng/ml, P <0.01). Interestingly, following LTA/PGN challenge of adipocytes, there was a significant decrease in adiponectin concentration in the culture medium (control: 20.3 ?0.78, LTA/PGN: 18.23 ?1.14 pg/ml, P = 0.016). Conclusions The proinflammatory but not anti-inflammatory components of the immune response (assessed by neutrophil recruitment and adiponectin concentrations, respectively) differed significantly between the age groups. Since adiponectin decreased on direct stimulation of adipocytes, the increased adiponectin within the lungs may reflect increased lung permeability, PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/27766426 and/or production by other cells within the lung. These findings are important in understanding the response to pulmonary infections in the older patient and may lead to the identification of novel targets for age-dependent therapeutic strategies.Corporation, Sagamihara, Japan). Auto anti-HMGB1 antibodies were detected in serum by a homemade ELISA test. Results Forty-two septic shock patients were included. Median age was 70 (59 to 78) years, SAPS 2 was 68 (51 to 83), and the mortality rate was 29 . HMGB1 was undetectable in the plasma of a control population. In contrast, high levels of HMGB1 were found in all septic patients (median = 5.71 ng/ml at D7). Pinometostat manufacturer Thirteen patients (38 ) presented a significant production of auto antiHMGB1 IgG antibodies during the course of sepsis. The age, sex ratio, median HMGB1 level and mortality rate were similar in patients producing (PPAb) and not producing antibodies (PNPAb). However, as compared with the PNPAb patients, the PPAb group had significant higher APACHE II (P = 0.027) and SOFA scores (P = 0.02) at the onset of shock and had a more important but nonsignificant decrease of the cardiovascular SOFA score between day 1 and day 7 (P = 0.063). Conclusions Naturally acquired anti-HMGB1 antibodies are produced during septic shock. The presence of antibodies is associated with higher severity scores and might be associated with an improvement of the haemodynamic dysfunction. The neutralizing capacity of these autoantibodies and their physiological role remain to be investigated.P56 Delayed increased S100A9 mRNA predicts hospital-acquired infection after septic shockM Fontaine1, A Pachot2, A Larue2, B Mougin2, C Landelle1, C Allombert2, F Venet3, M-A Cazalis2, G Monneret3, A Lepape1 1Intensive Care Units, Hospices Civils de Lyon, CH Lyon-Sud, Lyon, France; 2Joint Unit Hospices Civils de Lyon `bioMerieux, H ital E. Herriot, Lyon, France; 3Immunology Laboratory, Hospices Civils de Lyon, H ital E. Herriot, Lyon, France Critical Care 2009, 13(Suppl 4):P56 (doi: 10.1186/cc8126) Introduction Septic shock (SS) remains a serious disease with.