Es of both MK-1439 cost hepatic and renal CAT, POD and SOD as compared to control group. Treatment of SAME along with gentamicin increased the activity level of CAT, POD and SOD, dose dependently, as compared to the gentamicin treated group. However, non significant (P>0.05) change was recorded with the treatment of SAME alone as compared to control group.Effects of SAME on hepatorenal TBARS, GSH and DNA fragmentation in ratEffect of gentamicin and gentamicin plus SAME on urinary protein, glucose and urine output are shown inTable 6 and 7 show the effects of SAME on TBARS, GSH and DNA fragmentation in liver and renal samples.Table 2 Effects of SAME on serum chemistry in ratTreatment Total protein (mg/dl) Albumin (mg/dl) Triglycerides Total (mg/dl) cholesterol (mg/dl) 12.8 ?2.8A 18.6 ?2.0B 17.2 ?2.2B 6.8 ?0.84A 12.0 ?2.95D Total bilirubin (mg/dl) Direct bilirubin (mg/dl) Urea (mg/dl) Creatinine (mg/dl)Control (Saline, 0.9 NaCl) GTM (100 mg/kg bw) GTM + SAME (100 mg/kg bw) GTM + SAME (200 mg/kg bw)42.5 ?4.7A 22.7 ?3.5A 33.7 ?3.7D 16.4 ?2.3D 35.7 ?4.1C 18.4 ?2.8C 40.2 ?5.6B1.51 ?0.36A 0.87 ?0.14A 33.5 ?5.7A 2.83 ?0.45D 1.17 ?0.44E 56.2 ?6.6C0.78 ?0.16A 3.7 ?0.60C 2.6 ?0.65B 1.2 ?0.26A 0.84 ?0.32A10.1 ?1.73CD 1.69 ?0.34C 1.03 ?0.16C 43.2 ?5.2BC 8.8 ?1.50B 7.2 ?1.69A 1.67 ?0.24C 0.97 ?0.17B 40.6 ?6.2B 1.49 ?0.36A 0.83 ?0.16A 32.4 ?4.6A21.1 ?3.2AB 13.1 ?2.2A 12.1 ?1.4ASAME (200 mg/kg bw) 43.2 ?4.2A 23.2 ?2.9AMean ?SD (n = 06). A-D, Means not sharing common letters within a column are significantly (P < 0.01) different from each other. GTM, gentamicin; SAME, methanol extract of Sonchus asperKhan et al. BMC Complementary and Alternative Medicine 2011, 11:113 http://www.biomedcentral.com/1472-6882/11/Page 5 ofTable 3 Effects of SAME on urinary profile of ratTreatment Control (Saline, 0.9 PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/28192408 NaCl) GTM (100 mg/kg bw) GTM + SAME (100 mg/kg bw) GTM + SAME (200 mg/kg bw) SAME (200 mg/kg bw) Urinary volume (ml/day) 7.4 ?1.24A 14.6 ?3.24C 11.5 ?2.76B 9.3 ?2.22A 8.3 ?1.63A Urinary glucose (mg/L/day) 9.4 ?1.2A 53.8 ?7.4D 42.9 ?5.4C 25.5 ?3.4B 8.4 ?1.3A Urinary protein (mg/L/day) 16.5 ?2.62A 38.8 ?7.16B 28.9 ?4.32B 19.8 ?3.16A 16.31 ?4.25AMean ?SD (n = 06). A-D, Means not sharing common letters within a column are significantly (P < 0.01) different from each other. GTM, gentamicin; SAME, methanol extract of Sonchus asperAdministration of gentamicin significantly (P < 0.01) decreased the level of GSH while increased (P < 0.01) the level of TBARS and DNA fragmentation as compared to the control group. Administration of SAME in combination with gentamicin significantly reversed the level of GSH, TBARS and DNA injuries as compared to gentamicin treated group in a dose dependent manner in both the liver and kidney samples. Treatment of SAME alone did not cause any significant (P>0.05) change in the level of GSH, TBARS and DNA injuries in renal samples.Effects of SAME on DNA ladder assayat higher dose of SAME (200 mg/kg bw). Light microscopic examination of H E stained slides of the group having 200 mg/kg bw of SAME alone showed normal architecture of liver hepatic cords and plates radiating out from the central vein (Figure 2E).Histopathological studies of kidneysDNA ladder assay showed a continuous banding pattern of fragmented DNA induced with gentamicin in both the organs; liver and kidneys (Figure 1). DNA injuries were comprehensively eliminated by the SAME treatment in liver and kidney samples. However, DNA fragmentation was not recorded in the control samples of liver.