Ased efficiency in comparison with classical EVs. The regenerative properties of mechanically induced MSC-EVs were confirmed in vivo inside a murine model of chronic heart failure. Mice underwent surgery to induce a permanent myocardial infarction and had been treated 1 month postinfarction by percutaneous EV injection of 3 1011 EVs (higher and medium shear pressure EVs, serum starvation EVs) or MSCs (1 106). Heart functional parameters had been analysed by ultrasound two months postinfarction. Interestingly, shear-induced EVs had the exact same impact in comparison to EVs made by starvation or to parental cells, with an increase in the left ventricular ejection fraction of 100 in comparison to pretreatment values, whereas PBS injected controls lost 18 . Biomolecular analysis and histology are ongoing. Summary/conclusion: We demonstrated an equal or Caspase 14 Proteins Biological Activity superior regenerative impact of high-yield mechanically produced EVs compared to spontaneously released EVs or parental cells in vitro and in vivo. This special technologies for EV production combines decisive assets for clinical translation of EV-based regenerative medicine: a GMP-compliant setup, high-density cell culture, high-yield release of EVs per cell and highpurity EVs.PT08.Coronary artery bypass graft (CABG) surgery – Does the surgery procedure affect the EV status Christina Schlingschroeder1; Sebastian Borosch2; Christian Stoppe3; Eva Miriam Buhl4; Christian Beckers1; Ruediger Autschbach1; Sandra Kraemer1 Department of Thoracic and Cardiovascular Surgery, University Hospital RWTH Aachen, Aachen, Germany; 2Department of Thoracic and Cardiovascular Surgery, Uniklinik RWTH Aachen, Aachen, Germany; three Department of Intensive Care and Intermediate Care, University Hospital, RWTH Aachen, Aachen, Germany; 4Electron Microscopic Facility, Healthcare Faculty, University Hospital RWTH, Aachen, Aachen, GermanyPT08.High yield mechanically induced extracellular vesicles display as effective regenerative impact as their parental mesenchymal stem cells or starvation-induced EVs in vitro and in vivo Within a model of chronic heart MMP-8 Proteins web failure Iris Marangon1; Max Piffoux2; Nathalie Mougenot3; Claire Wilhelm2; Florence Gazeau2; Onnik Agbulut4; Amanda K A silvaBackground: Sufferers with high-grade stenosis of coronary arteries are frequently scheduled for coronary artery bypass graft (CABG) surgery, which is often performed either with (on pump) or with no (off pump) the usage of cardiopulmonary bypass (CPB). Recent research indicated thatThursday, 03 Maythe concentration of extracellular vesicles (EVs) increases in individuals undergoing an on-pump CABG surgery and these EVs is usually made use of as an early biomarker for myocardial ischemia. Within this context, it has come into focus if employing the CPB influences the post-operative EV status or when the increased vesicles rely only around the surgical trauma itself. We consequently analysed the EV composition, size and concentration in sufferers undergoing off pump CABG surgery. Solutions: EDTA-plasma from off pump CABG-patients at four time points (preoperative, instantly immediately after surgery, 24 h and 48 h postoperative) was centrifuged at 2000 g for ten min and also at 10,000 g for 20 min. The resulting supernatant was used to isolate EVs by size-exclusion chromatography (SEC). Particle size and concentration had been analysed by nanoparticle tracking analysis (NTA), their composition was determined by western blot. Electron microscopy was performed to make sure profitable EV isolation. Benefits: EVs from plasma had been effectively isol.