Roject representing each and every library described within the manuscript. The experiment accession
Roject representing each and every library described inside the manuscript. The experiment accession numbers are sequencial and range from SRX671492 to SRX671503. Moreover, added files supporting the outcomes of this short article have been uploaded to LabAchvives; these files are available employing the DOI: ten.6070/H4028PGQ.Extra filesAdditional file 1: Pairing statistics for cassava F3 and F5 Tags. Added file two: Manihot esculenta -147- annotated transcriptome_genes. More file three: List of all differentially expressed genes in T200 at 12 dpi. Extra file four: List of all differentially expressed genes in T200 at 32 dpi. Further file 5: List of all differentially expressed genes in T200 at 67 dpi. Further file 6: List of all differentially expressed genes in TME3 at 12 dpi. Additional file 7: List of all differentially expressed genes in TME3 at 32 dpi. Extra file eight: List of all differentially expressed genes in TME3 at 67 dpi. Additional file 9: Comparisons of number of differentially expressed genes among 12, 32 and 67 dpi in T200. Further file ten: Comparisons of quantity of differentially expressed genes involving 12, 32 and 67 dpi in TME3. Additional file 11: Transcript quantification for mock-inoculated T200 and TME3 leaf tissue. Additional file 12: Comparative analyses of Kegg metabolic pathways differentially expressed in SACMV-infected Arabidopsis, cassava T200 and TME3.Allie et al. BMC Genomics 2014, 15:1006 biomedcentral.com/1471-2164/15/Page 27 ofAdditional file 13: SACMV-responsive R gene homologues and histone-related genes in T200 and TME3. Added file 14: Primers employed for qPCR validations.Abbreviations DEG: Differentially expressed genes; dpi: Days post infection; CMD: Cassava mosaic disease; ET: Ethylene; HR: PI4KIIIβ Formulation Hypersensitive response; JA: Jasmonic acid; NGS: Subsequent generation sequencing; R: Resistant; SACMV: South African cassava mosaic virus; SA: Salicylic acid; S: Susceptible; TF: 5-HT7 Receptor Antagonist Species Transcription aspect. Competing interests The authors declare that they have no competing interests. Authors’ contributions FA carried out infectivity study and sample preparation of susceptible T200 samples, and annotated the DEGs from phyozome; EJP performed infectivity study and sample preparation for all resistant TME3 samples. MJO assembled and edited the sequencing reads also as carried out all subsequent Bioinformatic information analysis including the quantification of transcripts. FA and CR compiled and edited the manuscript. CR is principal investigator on the project and conceived the all round notion on the study. All authors study and authorized the final manuscript. Acknowledgments This project was sponsored by the National Investigation Foundation and also the National Bioinformatics Network. Contributions from Casquip Cassava Starch Manufacturing Pty. Ltd. SA are also appreciated. The authors also acknowledge the help of ETHZ and also the Functional Genomics Center in Zurich and would especially like to thank Martin Ryan for his initial contribution around the assembly and mapping of this transcriptome data. Author facts 1 College of Molecular and Cell Biology, University with the Witwatersrand, 1 Jan Smuts Ave, Braamfontein, Johannesburg 2000, South Africa. 2Functional Genomics Center, Zurich, UNI ETH Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland. Received: ten January 2014 Accepted: 23 October 2014 Published: 20 November 2014 References 1. FAO: Cassava for Meals and Energy Security. Rome: FAO Media Center; 2008. fao.org/New.