Briefly, right after fixation and permeabilization of the cells, the integrated BrdU was detected by an anti-BrdU-POD antibody adopted by incubation with Luminol. The total of sure antibody was quantified by resolve of relative mild models with a chemiluminescence plate
Merck KGaA, Darmstadt, Germany). Monoclonal rabbit anti-GPX1 (clone EPR3312) antibody for immunohistochemistry was obtained from Novus Europe (Cambridge, Uk), monoclonal mouse anti-sleek muscle mass a-actin (Clone 1A4) antibody for immunohistochemistry…