Ed by a two-way ANOVA followed by a post hoc Tukey’s test. Comparisons between two groups were performed using an unpaired Student’s t-test. A p-value of ,0.05 was considered to be statistically significant.IKKi deficiency enhances cardiac hypertrophic and dysfunctional responses to pressure overloadTo clarify the direct MedChemExpress 298690-60-5 relationship between IKKi deficiencymediated changes and cardiac hypertrophy, IKKi-KO mice and their WT littermates were subjected to cardiac pressure overload by AB or a sham surgery. The cumulative survival rate at 4 weeks after AB was strikingly decreased by IKKi deficiency (Figure 2E). Table 1. Anatomic and echocardiographic analysis of 24- to 30 -week-old IKKi KO mice and WT mice.Results IKKi expression is induced in hypertrophic hearts following ABTo investigate the potential role of IKKi in cardiac hypertrophy, we used the well-established cardiac hypertrophy model induced by AB. We found that IKKi protein and mRNA levels were slightly increased at 1 week 
but significantly up-regulated at 4 and 8 weeks after AB (Figure 1). These findings demonstrate that IKKi expression compensatorily increases during the development of cardiac hypertrophy.Parameter BW (g) HW/BW(mg/g) LW/BW(mg/g) HW/TL(mg/cm) HR (beats/min) LVEDD(mm) LVESD(mm) LVPWD (mm) FS ( )WT(n = 6) 30.3960.66 4.0560.09 4.6760.15 6.5460.24 501612 3.5360.05 2.0860.06 0.7260.01 41.2261.IKKi KO(n = 6) 31.0860.73 5.4360.22* 4.4460.16 9.1060.32* 520623 4.2560.11* 2.7860.12* 0.7360.02* 3461.18*IKKi deficiency induces severe and spontaneous hypertrophyTo evaluate alterations in cardiac structures and functions in IKKi-deficient mice, we harvested the hearts of 30-week-old mice and performed echocardiograms. It was documented that the HW, HW/BW, HW/TL, LVEDD, and LVESD were significantly increased and the FS was decreased compared with the WT mice (Table 1), which agrees with our hypothesis that the loss of IKKi leads to spontaneous cardiac hypertrophy and dysfunction.BW,body weight;HW/BW,heart weight/body weight;LW/BW,lung weight/body weight; HW/TL,heart weight/tibial length; HR,heart rate; LVEDD,left ventricular end-diastolic dimension; LVESD,left ventricular end-AKT inhibitor 2 systolic diameter; LVPWD,left ventricular posterior wall dimension; IVSD, Interventricular septal thickness at end-diastole; FS,fractional shortening. *P,0.05 vs WT/KO. doi:10.1371/journal.pone.0053412.tIKKi Deficiency Promotes Cardiac HypertrophyEchocardiographic analyses were also utilized to evaluate cardiac structures and functions, including the chamber diameter, wall thicknesses and function of the left ventricle. The KO and WT mice that underwent sham surgery did not differ echocardiographically. However, the echocardiographic measurements of LVEDD, LVESD, interventricular septal thickness at end-diastole (IVSD), left ventricular posterior wall thickness at end-diastole (LVPWD), and fractional shortening (FS) indicated deteriorated cardiac hypertrophy and dysfunction in the KO mice compared with the WT mice (Figure 2A). The LV hemodynamic parameters of the anesthetized mice that were obtained during the acquisition of the pressure-volume (PV) loop further confirmed this 
significantly deteriorated hemodynamic dysfunction (volume and systolic and diastolic function) 1326631 of the LV in the IKKi-KO mice as shown in Table 2. Under basal conditions, pressure-overloaded KO mice showed significantly increased ratios of HW/BW, HW/TL and LW/BW and cardiomyocyte cross-sectional area (CSA) compared with the WT mice.Ed by a two-way ANOVA followed by a post hoc Tukey’s test. Comparisons between two groups were performed using an unpaired Student’s t-test. A p-value of ,0.05 was considered to be statistically significant.IKKi deficiency enhances cardiac hypertrophic and dysfunctional responses to pressure overloadTo clarify the direct relationship between IKKi deficiencymediated changes and cardiac hypertrophy, IKKi-KO mice and their WT littermates were subjected to cardiac pressure overload by AB or a sham surgery. The cumulative survival rate at 4 weeks after AB was strikingly decreased by IKKi deficiency (Figure 2E). Table 1. Anatomic and echocardiographic analysis of 24- to 30 -week-old IKKi KO mice and WT mice.Results IKKi expression is induced in hypertrophic hearts following ABTo investigate the potential role of IKKi in cardiac hypertrophy, we used the well-established cardiac hypertrophy model induced by AB. We found that IKKi protein and mRNA levels were slightly increased at 1 week but significantly up-regulated at 4 and 8 weeks after AB (Figure 1). These findings demonstrate that IKKi expression compensatorily increases during the development of cardiac hypertrophy.Parameter BW (g) HW/BW(mg/g) LW/BW(mg/g) HW/TL(mg/cm) HR (beats/min) LVEDD(mm) LVESD(mm) LVPWD (mm) FS ( )WT(n = 6) 30.3960.66 4.0560.09 4.6760.15 6.5460.24 501612 3.5360.05 2.0860.06 0.7260.01 41.2261.IKKi KO(n = 6) 31.0860.73 5.4360.22* 4.4460.16 9.1060.32* 520623 4.2560.11* 2.7860.12* 0.7360.02* 3461.18*IKKi deficiency induces severe and spontaneous hypertrophyTo evaluate alterations in cardiac structures and functions in IKKi-deficient mice, we harvested the hearts of 30-week-old mice and performed echocardiograms. It was documented that the HW, HW/BW, HW/TL, LVEDD, and LVESD were significantly increased and the FS was decreased compared with the WT mice (Table 1), which agrees with our hypothesis that the loss of IKKi leads to spontaneous cardiac hypertrophy and dysfunction.BW,body weight;HW/BW,heart weight/body weight;LW/BW,lung weight/body weight; HW/TL,heart weight/tibial length; HR,heart rate; LVEDD,left ventricular end-diastolic dimension; LVESD,left ventricular end-systolic diameter; LVPWD,left ventricular posterior wall dimension; IVSD, Interventricular septal thickness at end-diastole; FS,fractional shortening. *P,0.05 vs WT/KO. doi:10.1371/journal.pone.0053412.tIKKi Deficiency Promotes Cardiac HypertrophyEchocardiographic analyses were also utilized to evaluate cardiac structures and functions, including the chamber diameter, wall thicknesses and function of the left ventricle. The KO and WT mice that underwent sham surgery did not differ echocardiographically. However, the echocardiographic measurements of LVEDD, LVESD, interventricular septal thickness at end-diastole (IVSD), left ventricular posterior wall thickness at end-diastole (LVPWD), and fractional shortening (FS) indicated deteriorated cardiac hypertrophy and dysfunction in the KO mice compared with the WT mice (Figure 2A). The LV hemodynamic parameters of the anesthetized mice that were obtained during the acquisition of the pressure-volume (PV) loop further confirmed this significantly deteriorated hemodynamic dysfunction (volume and systolic and diastolic function) 1326631 of the LV in the IKKi-KO mice as shown in Table 2. Under basal conditions, pressure-overloaded KO mice showed significantly increased ratios of HW/BW, HW/TL and LW/BW and cardiomyocyte cross-sectional area (CSA) compared with the WT mice.