Gth a1-syntrophin cDNA (see above) as template and primers 59-ACTGGAATTCCGCCGCGTGACGGTGCGCAAGGC-
Gth a1-syntrophin cDNA (see above) as template and primers 59-ACTGGAATTCCGCCGCGTGACGGTGCGCAAGGC-39 and 59ATCGCTCGAGCTTCATGTACTTAACCTCCAACACAACCTCCTTGCCTGTCTTC-39. The resulting PCR fragment was inserted by blunt end ligation into the EcoRV site of pBluescript (Fermentas, Glen…