Nections, including those mediated by differences in cell adhesion molecules, are likely to help underpin individual differences in addiction and cognitive phenotypes [38].Supporting InformationS1 Table. Primer sequences used for SNP genotyping using Sequenom panels and for the simple sequence repeat annotated as rs71534387. (XLSX) S2 Table. Data and statistical analysis from mouse behavioral tests. Table A: JNJ-26481585 biological activity Original spreadsheet and statistical analysis of the 60 min locomotion in the original CSMD1 line. Mice were injected with saline or 10 mg/kg s.c. cocaine, as indicated and immediately placed into dark, sound-attenuating locomotor boxes (42 x 42 cm). Total distance traveled was calculated from infrared beam breaks by an Optovarimax ATS System. Table B: Original spreadsheet and statistical analysis of the Rotarod test in the original CSMD1 line. Latencies (s) to fall off on each day are presented. Table C: Original spreadsheet and statistical analysis of the weights of the back-crossed CSMD1 mice. Table D: Original spreadsheet and statistical analysis of the hanging wire test of the back-crossed CSMD1 mice. Table E: Original spreadsheet and statistical analysis of the Rotarod test of the back-crossed CSMD1 mice. Latencies (s) to fall off on each day are presented. Table F: Original spreadsheet and statistical analysis of the Dark box emergence and the open field tests of the back-crossed CSMD1 mice. For the Dark box emergence test, the testing cage (18 x 36 cm) consisted of two compartments: a dark chamber (18 x 18 cm) with black walls and a small opening (5 cm) leading to a Plexiglas compartment. An animal was placed into the dark chamber. The latency to emerge and the time spent outside the dark chamber during the 10 min trial were measured using the Optovarimax system (Columbus Instruments, OH). For the Open Field test, mice were placed singly in an open field (42 x 42 cm) for ten minutes. The time each animal spent in the central quadrant was recorded. Table G: Original spreadsheet and statistical analysis of the Conditioned Place preference (CPP) test of the back-crossed CSMD1 mice. Times spent on the cocaine-paired side during two pre-tests (Wire-T1, Wire-T2) and post-test (Wire-T3) and the difference (Ave T) between the post-test and average of the two pretests (Ave T 1?) are presented. Table H: Original spreadsheet and statistical analysis of the locomotion WP1066 price recorded during the CPP pre-test (T1- Loco) of the back-crossed CSMD1 mice. Table I: Original spreadsheet and statistical analysis of the locomotion recorded during the first CPP conditioning session (1st COC Loco) with the back-crossed CSMD1 mice. Table J: Original spreadsheet and statistical analysis of thePLOS ONE | DOI:10.1371/journal.pone.0120908 July 14,13 /CSMD1 Variants and Addictionlocomotor sensitiziation of the back-crossed CSMD1 mice that occurred during the two CPP conditioning sessions (1st COC, 2nd COC). Table K: Original spreadsheet and statistical analysis of the Morris water maze (MWM) learning curves of the back-crossed CSMD1 mice. Values for each day are averages of four trials performed on that day. Table L: Original spreadsheet and statistical analysis of the Morris water maze (MWM) Latencies to reach the platform during each trial on day 1 are presented. Table M: Original spreadsheet and statistical analysis of the results from the (MWM) probe trial. (XLSX)AcknowledgmentsThis work was supported by the National Institutes of Health (NIH) ntramural Res.Nections, including those mediated by differences in cell adhesion molecules, are likely to help underpin individual differences in addiction and cognitive phenotypes [38].Supporting InformationS1 Table. Primer sequences used for SNP genotyping using Sequenom panels and for the simple sequence repeat annotated as rs71534387. (XLSX) S2 Table. Data and statistical analysis from mouse behavioral tests. Table A: Original spreadsheet and statistical analysis of the 60 min locomotion in the original CSMD1 line. Mice were injected with saline or 10 mg/kg s.c. cocaine, as indicated and immediately placed into dark, sound-attenuating locomotor boxes (42 x 42 cm). Total distance traveled was calculated from infrared beam breaks by an Optovarimax ATS System. Table B: Original spreadsheet and statistical analysis of the Rotarod test in the original CSMD1 line. Latencies (s) to fall off on each day are presented. Table C: Original spreadsheet and statistical analysis of the weights of the back-crossed CSMD1 mice. Table D: Original spreadsheet and statistical analysis of the hanging wire test of the back-crossed CSMD1 mice. Table E: Original spreadsheet and statistical analysis of the Rotarod test of the back-crossed CSMD1 mice. Latencies (s) to fall off on each day are presented. Table F: Original spreadsheet and statistical analysis of the Dark box emergence and the open field tests of the back-crossed CSMD1 mice. For the Dark box emergence test, the testing cage (18 x 36 cm) consisted of two compartments: a dark chamber (18 x 18 cm) with black walls and a small opening (5 cm) leading to a Plexiglas compartment. An animal was placed into the dark chamber. The latency to emerge and the time spent outside the dark chamber during the 10 min trial were measured using the Optovarimax system (Columbus Instruments, OH). For the Open Field test, mice were placed singly in an open field (42 x 42 cm) for ten minutes. The time each animal spent in the central quadrant was recorded. Table G: Original spreadsheet and statistical analysis of the Conditioned Place preference (CPP) test of the back-crossed CSMD1 mice. Times spent on the cocaine-paired side during two pre-tests (Wire-T1, Wire-T2) and post-test (Wire-T3) and the difference (Ave T) between the post-test and average of the two pretests (Ave T 1?) are presented. Table H: Original spreadsheet and statistical analysis of the locomotion recorded during the CPP pre-test (T1- Loco) of the back-crossed CSMD1 mice. Table I: Original spreadsheet and statistical analysis of the locomotion recorded during the first CPP conditioning session (1st COC Loco) with the back-crossed CSMD1 mice. Table J: Original spreadsheet and statistical analysis of thePLOS ONE | DOI:10.1371/journal.pone.0120908 July 14,13 /CSMD1 Variants and Addictionlocomotor sensitiziation of the back-crossed CSMD1 mice that occurred during the two CPP conditioning sessions (1st COC, 2nd COC). Table K: Original spreadsheet and statistical analysis of the Morris water maze (MWM) learning curves of the back-crossed CSMD1 mice. Values for each day are averages of four trials performed on that day. Table L: Original spreadsheet and statistical analysis of the Morris water maze (MWM) Latencies to reach the platform during each trial on day 1 are presented. Table M: Original spreadsheet and statistical analysis of the results from the (MWM) probe trial. (XLSX)AcknowledgmentsThis work was supported by the National Institutes of Health (NIH) ntramural Res.