Pecificity of over 99 ). If the test was reactive, the pharmacists informed about the need of a confirmatory test as soon as possible and provided Enzastaurin site information about the reference centre and NGOs for social and psychological support. More in-depth information about the testing process and the confirmation pathways on each programme has been published elsewhere.[18?0] Information on sociodemographic data, risk behaviours, HIV testing history and reasons for performing the test was collected from each participant through a paper questionnaire as part of the everyday routine and programme evaluation. The Castilla y Leon and the Basque programme shared very similar questionnaires. The one used in Catalonia was simpler and it was changed at midterm. Thus, not all variables were available since the beginning. The whole testing process took about 30 minutes in the case of a negative test. When the test was reactive, the time spent depended on the needs of each person. The three programmes were launched as a partnership between the Regional Ministries of Health and the Councils of Professional Associations of Pharmacists of each region, granting ethical and legal approval and following closely the Spanish data protection law. All participants Isoarnebin 4 site signed a written informed consent. Identifiers for both participating individuals and pharmacies were not recorded within the questionnaires. Thus, all the datasets were completely anonymous. The analysis executed using individual records were performed at regional level and in the context of public health responsibility. For the current study only aggregated dataPLOS ONE | DOI:10.1371/journal.pone.0134631 August 6,4 /In-Pharmacy Rapid HIV Testing. Programmes Evaluationfrom each programme were used. For all these reasons, approval by pnas.1408988111 the Ethics Committee was not required. A descriptive analysis of the participants’ characteristics was performed stratifying the results by gender/sexual behaviour: men who have sex with men (MSM), men who have sex with women (MSW) and women. In the event of men who did not provide enough information to classify them in this manner, they were included only within the total of participants. The results of each programme were compared with those of the regional surveillance system on new diagnosis from each region: the autonomous community of Castilla y Leon, the Basque Country and the provinces jir.2014.0227 of Tarragona and Barcelona excluding results from the city of Barcelona. For this comparison, we took into account data from the years in which the programme was implemented. Statistical significance was analysed using the 2 test for categorical variables or the Fisher’s exact test when indicated. The precision of the estimates for HIV prevalence were analysed by calculating the 95 confidence interval (95 CI).ResultsAltogether, 24151 people were tested in the three programmes and 226 had a reactive test result. The main characteristics of the participants and of those with a reactive result can be seen in Table 1. Approximately 46.0 of those who underwent testing were MSW whereas 52.2 of those with a reactive result were MSM. The proportion of Latin Americans was three times higher among those with a reactive result (15.5 ) than among the total of participants (5.2 ) (p<0.0001). Both the attendees and those with a reactive result in the programme from Castilla y Le were younger than those in the other programmes (p<0.0001 when comparing with the other participants and p = 0.02 when co.Pecificity of over 99 ). If the test was reactive, the pharmacists informed about the need of a confirmatory test as soon as possible and provided information about the reference centre and NGOs for social and psychological support. More in-depth information about the testing process and the confirmation pathways on each programme has been published elsewhere.[18?0] Information on sociodemographic data, risk behaviours, HIV testing history and reasons for performing the test was collected from each participant through a paper questionnaire as part of the everyday routine and programme evaluation. The Castilla y Leon and the Basque programme shared very similar questionnaires. The one used in Catalonia was simpler and it was changed at midterm. Thus, not all variables were available since the beginning. The whole testing process took about 30 minutes in the case of a negative test. When the test was reactive, the time spent depended on the needs of each person. The three programmes were launched as a partnership between the Regional Ministries of Health and the Councils of Professional Associations of Pharmacists of each region, granting ethical and legal approval and following closely the Spanish data protection law. All participants signed a written informed consent. Identifiers for both participating individuals and pharmacies were not recorded within the questionnaires. Thus, all the datasets were completely anonymous. The analysis executed using individual records were performed at regional level and in the context of public health responsibility. For the current study only aggregated dataPLOS ONE | DOI:10.1371/journal.pone.0134631 August 6,4 /In-Pharmacy Rapid HIV Testing. Programmes Evaluationfrom each programme were used. For all these reasons, approval by pnas.1408988111 the Ethics Committee was not required. A descriptive analysis of the participants’ characteristics was performed stratifying the results by gender/sexual behaviour: men who have sex with men (MSM), men who have sex with women (MSW) and women. In the event of men who did not provide enough information to classify them in this manner, they were included only within the total of participants. The results of each programme were compared with those of the regional surveillance system on new diagnosis from each region: the autonomous community of Castilla y Leon, the Basque Country and the provinces jir.2014.0227 of Tarragona and Barcelona excluding results from the city of Barcelona. For this comparison, we took into account data from the years in which the programme was implemented. Statistical significance was analysed using the 2 test for categorical variables or the Fisher’s exact test when indicated. The precision of the estimates for HIV prevalence were analysed by calculating the 95 confidence interval (95 CI).ResultsAltogether, 24151 people were tested in the three programmes and 226 had a reactive test result. The main characteristics of the participants and of those with a reactive result can be seen in Table 1. Approximately 46.0 of those who underwent testing were MSW whereas 52.2 of those with a reactive result were MSM. The proportion of Latin Americans was three times higher among those with a reactive result (15.5 ) than among the total of participants (5.2 ) (p<0.0001). Both the attendees and those with a reactive result in the programme from Castilla y Le were younger than those in the other programmes (p<0.0001 when comparing with the other participants and p = 0.02 when co.