He MCF7-si cells, the maximal induction of the G2 block
He MCF7-si cells, the maximal induction of the G2 block was lower than that in MCF7-wt cells (57.8 ?1.5 vs. 63.2 ?1.1 ). These results demonstrate that without affecting the proliferation, the clonogenic survival and the membrane integrity of MCF7 cells, the knockdown of PP2C does reflect, at least to some extent, on their cell cycle distribution.Tumorigenicity of wild type and PP2C knockdown MCF7 cells To address the involvement of PP2C in tumorigenesis, 1 * 107 MCF-wt and MCF7-si cells were inoculated subcuta-Page 6 of2007, :http://www.molecular-cancer.com/content/6/1/PNPPMedChemExpress PNPP Figure 3 of the chemosensitivity of wild type and PP2C siRNA-expressing MCF7 cells Evaluation Evaluation of PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/28945807 the chemosensitivity of wild type and PP2C siRNA-expressing MCF7 cells. A and B: Effect of different doses of doxorubicin on the proliferation of MCF7-wt and MCF7-si cells. The cells were either exposed to the drug for 24 h (A) or continuously (B). Values represent average ?SD (n = 3). C: Effect of doxorubicin on the clonogenicity of MCF7-wt and MCF7-si cells. Values represent average ?SD (n = 3). D: Effect of gemcitabine on the clonogenicity of MCF7-wt and MCF7-si cells. Values represent average ?SD (n = 3). E and F: Quantification of the effect of doxorubicin on the viability (i.e. the membrane integrity) of MCF7-wt and MCF7-si cells. The viability was determined by means of FACS analysis. Values represent average ?SD (n = 3). G: Representative images of the cell cycle distribution of MCF7-wt and MCF7-si cells after 48 h of doxorubicin treatment.neously (s.c.) into both hind limbs of immunodeficient nude mice. Neither the MCF7-wt cells, however, nor the MCF7-si cells turned out to be able to develop tumors under physiological conditions (Table 4). Based on thenotion that MCF7 cells are hormone-dependent breast cancer cells, and on the assumption that their in vivo growth therefore likely depends on the levels of circulating hormones, we next implanted estrogen-containingPage 7 of2007, :http://www.molecular-cancer.com/content/6/1/Table 3: Cell cycle analysis of wild type and PP2C siRNA-expressing MCF7 cells upon treatment with chemotherapy.Time (h)Dox Dose ( M) 0 1 2.5 5 10 20 0 1 2.5 5 10 20 0 1 2.5 5 10G0/GMCF7-wt SG2/MG0/GMCF7-si SG2/M53.6 ?0.5 74.8 ?0.9 50.4 ?0.7 45.1 ?0.2 38.9 ?1.3 40.8 ?1.8 61.5 ?0.6 71.4 ?3.5 35.7 ?1.3 31.7 ?0.5 30.3 ?1.0 33.2 ?1.8 71.9 ?1.7 52.5 ?7.2 46.4 ?1.2 38.8 ?0.3 34.1 ?0.7 32.8 ?2.33.3 ?1.2 8.0 ?0.6 4.1 ?0.8 6.4 ?0.2 29.5 ?0.8 31.3 ?2.5 29.1 ?1.0 8.9 ?1.7 3.9 ?0.3 5.2 ?1.6 23.6 ?0.8 48.6 ?7.7 22.8 ?0.7 3.1 ?1.9 0.5 ?0.5 0.1 ?0.1 16.4 ?1.7 39.9 ?1.13.1 ?0.3 17.2 ?0.3 45.5 ?1.5 48.5 ?0.1 31.6 ?0.8 27.8 ?1.3 9.4 ?0.7 19.7 ?5.2 60.4 ?1.0 63.2 ?1.1 46.1 ?0.7 18.3?9.4 5.4 ?1.3 44.4 ?8.9 53.3 ?2.0 61.3 ?0.3 49.5 ?2.3 27.3 ?1.56.6 ?0.5 * 53.9 ?0.6 * 52.1 ?0.9 51.5 ?1.6 * 41.3 ?0.3 * 54.9 ?1.4 * 61.2 ?1.8 52.2 ?2.7 * 58.3 ?5.8 * 49.6 ?1.0 * 44.0 ?1.7 * 50.3 ?0.8 * 59.2 ?0.9 * 47.4 ?7.3 41.3 ?2.6 * 40.4 ?0.8 * 37.5 ?1.5 * N.Q.29.7 ?0.6 * 30.7 ?1.0 * 5.0 ?2.7 5.1 ?2.4 32.5 ?1.6 * 30.2 ?0.5 29.5 ?1.7 2.5 ?1.2 * 3.2 ?0.6 2.5 ?0.7 8.4 ?0.6 * 43.4 ?2.4 30.4 ?1.6 * 0.5 ?0.9 1.9 ?3.0 1.8 ?1.6 3.5 ?2.5 * N.Q.13.7 ?0.9 15.4 ?1.3 42.8 ?1.9 43.4 ?0.9 * 26.2 ?1.5 * 14.9 ?1.4 * 11.2 ?0.1 * 45.3 ?1.7 * 38.5 ?5.3 * 47.8 ?0.4 * 47.6 ?2.3 6.4 ?1.6 10.5 ?0.6 * 52.1 ?8.2 56.8 ?1.8 57.8 ?1.5 * 57.1 ?2.6 * N.Q.Multicycle algorithm-based quantification of the amounts of MCF7-wt and MCF7-si cells in the G0/G1, the S and the G2/M phase of the cell cycle at 24, 48 a.