Submitted to the NCBI Gene Expression Omnibus (GEO; http://www.ncbi.
Submitted to the NCBI Gene Expression Omnibus (GEO; http://www.ncbi.nlm.nih.gov/geo/) under accession number GSE94014. A searchable database of our fetal brain epigenomics data PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/27872238 is available at http://www.epigenomicslab.com/onlinedata-resources. Authors’ contributions JM and NB conceived and supervised the study. JM obtained funding. HS performed the laboratory work, data analysis and bioinformatics. EH provided analytical support and performed analysis. LS provided analytical support and developed the website database resource. HS, EH and JM drafted the manuscript. All of the authors read and approved the final submission. Ethics approval and consent to participate Ethical approval for the HDBR was granted by the Royal Free Hospital research ethics committee under reference 08/H0712/34 and Human Tissue Authority (HTA) material storage license 12,220; ethical approval for the MRC Brain Bank was granted under reference 08/MRE09/38. Consent for publication Not applicable. Competing interests The authors declare that they have no competing interests.8.9.10.11.12.13.14.15.16.17.Publisher’s NoteSpringer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.18.19. Author details 1 Anlotinib web Department of Physics, University of Oxford, Oxford OX1 3RH, UK. 2 University of Exeter Medical School, RILD Building, Royal Devon and Exeter Hospital, Barrack Road, Exeter EX2 5DW, UK. 3School of Biological Sciences, University of Essex, Colchester CO4 3SQ, UK. 4MRC Centre for Neuropsychiatric Genetics and Genomics, Cardiff University School of Medicine, Cardiff CF24 4HQ, UK. Received: 4 May 2017 Accepted: 25 August20. 21.22.References 1. Vaquerizas JM, Kummerfeld SK, Teichmann SA, Luscombe NM. A census of human transcription factors: function, expression and evolution. Nat Rev Genet. 2009;10:252?3. 2. Lister R, Mukamel EA, Nery JR, Urich M, Puddifoot CA, Johnson ND, Lucero J, Huang Y, Dwork AJ, Schultz MD, et al. Global epigenomic reconfiguration during mammalian brain development. Science. 2013;341:1237905. 3. Spiers H, Hannon E, Schalkwyk LC, Smith R, Wong CC, O’Donovan MC, Bray NJ, Mill J. Methylomic trajectories across human fetal brain development. Genome Res. 2015;25:338?2. 4. Tahiliani M, Koh KP, Shen Y, Pastor WA, Bandukwala H, Brudno Y, Agarwal S, Iyer LM, Liu DR, Aravind L, Rao A. Conversion of 5-methylcytosine to 5hydroxymethylcytosine in mammalian DNA by MLL partner TET1. Science. 2009;324:930?.23. 24.25.26.27.Chen T, Ueda Y, Dodge JE, Wang Z, Li E. Establishment and maintenance of genomic methylation patterns in mouse embryonic stem cells by Dnmt3a and Dnmt3b. Mol Cell Biol. 2003;23:5594?05. Kriaucionis S, Heintz N. The nuclear DNA base 5-hydroxymethylcytosine is present in Purkinje neurons and the brain. Science. 2009;324:929?0. Khare T, Pai S, Koncevicius K, Pal M, Kriukiene E, Liutkeviciute Z, Irimia M, Jia P, Ptak C, Xia M, et al. 5-hmC in the brain is abundant in synaptic genes and shows differences at the exon-intron boundary. Nat Struct Mol Biol. 2012;19:1037?3. Spruijt CG, Gnerlich F, Smits AH, Pfaffeneder T, Jansen PW, Bauer C, Munzel M, Wagner M, Muller M, Khan F, et al. Dynamic readers for 5(hydroxy)methylcytosine and its oxidized derivatives. Cell. 2013;152:1146?9. Iurlaro M, Ficz G, Oxley D, Raiber EA, Bachman M, Booth MJ, Andrews S, Balasubramanian S, Reik W. A screen for hydroxymethylcytosine and formylcytosine binding proteins suggests functions in transcription and chromatin regul.