Hromosome pairs examined chromosome pairs examinedDNA methylation in B.distachyon chromosomesFig.
Hromosome pairs examined chromosome pairs examinedDNA methylation in B.distachyon chromosomesFig.DNA methylation C.I. 42053 cost patterns on chromosomes Bd and Bd of B.distachyon.a FISH with BAC clones ABRH, ABRH, ABRE (red fluorescence).b Distribution of MeC signals on the same chromosomes.c MeC foci distribution along the longitudinal axes of very condensed chromosome pair Bd excised from the metaphase spread shown on a .e MeC signal distribution of Bdhomologues with visible satellite area.g MeC foci arrangement of Bd homologues.Profiles, idiograms and chromosomes Bd d are oriented with their lengthy arm towards the left.Dark green tints on idiograms reflect low methylation level.Methylation profile descriptions as for Fig..DAPI counterstaining, blue fluorescence.Bars mN.Borowska et al.Fig.DNA methylation patterns on mitotic B.distachyon chromosomes just after AzaC therapy.a prometaphase chromosomes subjected to .mmolL AzaC.b Methylation pattern with the identical chromosomes.Positions of centromeres are pointed out by arrows.d FISH with BAC clones ABRH, ABRD and ABRC (red fluorescence) on metaphase chromosomes subjected to .mmolL AzaC.eDistribution of MeC foci around the similar chromosomes.g Prophaseprometaphase chromosomes following .mmolL AzaC remedy.h Methylation pattern from the same chromosomes.c, f, i Superimposed photos of DAPI stained chromosomes and signals of MeC residues.The arrows colour coding redvery high; yellowhigh and whitelow methylation level.DAPI counterstaining, blue fluorescence.Bars mrDNA internet site is localised proximally inside the long arm of chromosome Bd, while a nucleolar organising region (i.e.containing transcriptionally active S rDNA loci) is identified distally within the short arm of chromosome Bd (Draper et al.; Garvin et al).Unlike the preceding group, these chromosomes demonstrate more particular patterns of DNA methylation.Two common kinds of MeC foci distribution wereapparent for chromosome Bd, according to condensation, one for highly condensed chromosomes (Fig.a) and a further one for all those with clearly visible satellite regions (Fig.e).Each have been characterised by the highest levels of DNA methylation in pericentromeric regions, which abruptly decreased towards both chromosome termini.The methylation profile observed in much less condensed Bd chromosomesDNA methylation in B.distachyon chromosomesFig.Diverse demethylation of unique B.distachyon chromosomes subjected to .mmolL AzaC.a DAPIstained chromosomes.b Distribution of MeC residues.cSuperimposed photos of DAPI stained chromosomes and mC distribution.Arrow colour coding as for Fig..Bar mshowed considerably decrease methylation at S rDNA web sites (Fig.e) than within the very condensed chromosomes (Fig.c).The methylation pattern of chromosome Bd revealed two characteristic peaks of highdensity MeC foci (Fig.g).The first corresponded together with the pericentromeric regions of your chromosome whilst the second was positioned interstitially around the long arm.Decrease in intensity of antiMeC signals in proximal regions of chromosomes Bd was observed.Impact of AzaC on DNA methylation No prominent differences in antiMeC signal distribution had been observed in B.distachyon chromosome complements in the material subjected to the lowest (.mmolL) concentration of AzaC.Immunolocalisation of MeC in metacentric chromosome pairs showed PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/21308498 strong similarity to methylation patterns discovered in chromosomes of the nontreated material (Fig.a).The certain DNA methylation patterns of the smallest submetacentric pairs BdBd had been also retained.In.