Hromosome pairs examined chromosome pairs examinedDNA methylation in B.distachyon chromosomesFig.
Hromosome pairs examined chromosome pairs examinedDNA methylation in B.distachyon chromosomesFig.DNA methylation patterns on chromosomes Bd and Bd of B.distachyon.a FISH with BAC CAY10505 Purity & Documentation clones ABRH, ABRH, ABRE (red fluorescence).b Distribution of MeC signals around the very same chromosomes.c MeC foci distribution along the longitudinal axes of highly condensed chromosome pair Bd excised from the metaphase spread shown on a .e MeC signal distribution of Bdhomologues with visible satellite region.g MeC foci arrangement of Bd homologues.Profiles, idiograms and chromosomes Bd d are oriented with their extended arm towards the left.Dark green tints on idiograms reflect low methylation level.Methylation profile descriptions as for Fig..DAPI counterstaining, blue fluorescence.Bars mN.Borowska et al.Fig.DNA methylation patterns on mitotic B.distachyon chromosomes immediately after AzaC treatment.a prometaphase chromosomes subjected to .mmolL AzaC.b Methylation pattern on the very same chromosomes.Positions of centromeres are pointed out by arrows.d FISH with BAC clones ABRH, ABRD and ABRC (red fluorescence) on metaphase chromosomes subjected to .mmolL AzaC.eDistribution of MeC foci around the similar chromosomes.g Prophaseprometaphase chromosomes soon after .mmolL AzaC remedy.h Methylation pattern from the exact same chromosomes.c, f, i Superimposed photos of DAPI stained chromosomes and signals of MeC residues.The arrows colour coding redvery high; yellowhigh and whitelow methylation level.DAPI counterstaining, blue fluorescence.Bars mrDNA website is localised proximally inside the long arm of chromosome Bd, when a nucleolar organising region (i.e.containing transcriptionally active S rDNA loci) is identified distally in the brief arm of chromosome Bd (Draper et al.; Garvin et al).As opposed to the earlier group, these chromosomes demonstrate more distinct patterns of DNA methylation.Two general sorts of MeC foci distribution wereapparent for chromosome Bd, depending on condensation, one for highly condensed chromosomes (Fig.a) and an additional a single for all those with clearly visible satellite regions (Fig.e).Both were characterised by the highest levels of DNA methylation in pericentromeric regions, which abruptly decreased towards both chromosome termini.The methylation profile observed in significantly less condensed Bd chromosomesDNA methylation in B.distachyon chromosomesFig.Diverse demethylation of specific B.distachyon chromosomes subjected to .mmolL AzaC.a DAPIstained chromosomes.b Distribution of MeC residues.cSuperimposed images of DAPI stained chromosomes and mC distribution.Arrow colour coding as for Fig..Bar mshowed significantly decrease methylation at S rDNA web sites (Fig.e) than within the extremely condensed chromosomes (Fig.c).The methylation pattern of chromosome Bd revealed two characteristic peaks of highdensity MeC foci (Fig.g).The initial corresponded together with the pericentromeric regions of your chromosome when the second was positioned interstitially around the extended arm.Lower in intensity of antiMeC signals in proximal regions of chromosomes Bd was observed.Effect of AzaC on DNA methylation No prominent variations in antiMeC signal distribution have been observed in B.distachyon chromosome complements from the material subjected for the lowest (.mmolL) concentration of AzaC.Immunolocalisation of MeC in metacentric chromosome pairs showed PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/21308498 sturdy similarity to methylation patterns identified in chromosomes from the nontreated material (Fig.a).The distinct DNA methylation patterns in the smallest submetacentric pairs BdBd were also retained.In.