Hromosome pairs examined chromosome pairs examinedDNA methylation in B.distachyon chromosomesFig.
Hromosome pairs examined chromosome pairs examinedDNA methylation in B.distachyon chromosomesFig.DNA methylation patterns on chromosomes Bd and Bd of B.distachyon.a FISH with BAC clones ABRH, ABRH, ABRE (red fluorescence).b Distribution of MeC signals around the exact same chromosomes.c MeC foci distribution along the longitudinal axes of highly condensed chromosome pair Bd excised from the metaphase spread shown on a .e MeC signal distribution of Bdhomologues with visible satellite area.g MeC foci arrangement of Bd homologues.Profiles, idiograms and chromosomes Bd d are oriented with their long arm to the left.Dark green tints on idiograms Trovirdine Inhibitor reflect low methylation level.Methylation profile descriptions as for Fig..DAPI counterstaining, blue fluorescence.Bars mN.Borowska et al.Fig.DNA methylation patterns on mitotic B.distachyon chromosomes right after AzaC therapy.a prometaphase chromosomes subjected to .mmolL AzaC.b Methylation pattern in the exact same chromosomes.Positions of centromeres are pointed out by arrows.d FISH with BAC clones ABRH, ABRD and ABRC (red fluorescence) on metaphase chromosomes subjected to .mmolL AzaC.eDistribution of MeC foci around the exact same chromosomes.g Prophaseprometaphase chromosomes just after .mmolL AzaC therapy.h Methylation pattern on the exact same chromosomes.c, f, i Superimposed photos of DAPI stained chromosomes and signals of MeC residues.The arrows colour coding redvery higher; yellowhigh and whitelow methylation level.DAPI counterstaining, blue fluorescence.Bars mrDNA web site is localised proximally within the extended arm of chromosome Bd, even though a nucleolar organising region (i.e.containing transcriptionally active S rDNA loci) is discovered distally inside the brief arm of chromosome Bd (Draper et al.; Garvin et al).As opposed to the previous group, these chromosomes demonstrate a lot more specific patterns of DNA methylation.Two common types of MeC foci distribution wereapparent for chromosome Bd, depending on condensation, a single for very condensed chromosomes (Fig.a) and yet another 1 for all those with clearly visible satellite regions (Fig.e).Each were characterised by the highest levels of DNA methylation in pericentromeric regions, which abruptly decreased towards both chromosome termini.The methylation profile observed in significantly less condensed Bd chromosomesDNA methylation in B.distachyon chromosomesFig.Different demethylation of particular B.distachyon chromosomes subjected to .mmolL AzaC.a DAPIstained chromosomes.b Distribution of MeC residues.cSuperimposed images of DAPI stained chromosomes and mC distribution.Arrow colour coding as for Fig..Bar mshowed considerably reduced methylation at S rDNA web pages (Fig.e) than in the highly condensed chromosomes (Fig.c).The methylation pattern of chromosome Bd revealed two characteristic peaks of highdensity MeC foci (Fig.g).The very first corresponded using the pericentromeric regions of your chromosome while the second was situated interstitially around the long arm.Decrease in intensity of antiMeC signals in proximal regions of chromosomes Bd was observed.Impact of AzaC on DNA methylation No prominent variations in antiMeC signal distribution have been observed in B.distachyon chromosome complements in the material subjected for the lowest (.mmolL) concentration of AzaC.Immunolocalisation of MeC in metacentric chromosome pairs showed PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/21308498 sturdy similarity to methylation patterns identified in chromosomes in the nontreated material (Fig.a).The certain DNA methylation patterns of the smallest submetacentric pairs BdBd had been also retained.In.