Ted caspase staining (Figure figure supplement G) All constructive markers in the cell death method (McCall,).We noted that both DAPI and TUNEL were readily detected in apoptotic stage nurse cells in each exposed and unexposed femalesKacsoh et al.eLife ;e..eLife.ofResearch articleCell biology NeuroscienceFigure .Teacher flies can teach various batches of students.(A) Standard exposure setup for teachers teaching a number of batches of students.(B and C) Percent of eggs laid normalized to unexposed.(B) CantonS flies unexposed or exposed to wasps and paired with principal and secondary HisGFP students.(C) HisGFP flies unexposed or exposed to wasps and paired with primary and secondary CantonS students.For (B) and (C), error bars represent normal error (n biological replicates) (p .e)..eLife.Kacsoh et al.eLife ;e..eLife.ofResearch articleCell biology NeuroscienceFigure .Stagespecific apoptosis observed in waspexposed teachers and teacherexposed student flies.(A and B) Typical percent of apoptotic events for stage egg chambers.(A) CantonS exposed and unexposed ovary apoptosis.(B) HisGFP exposed and unexposed ovary apoptosis.(C to D) CantonS unexposedexposed ovariole.(E to F) HisGFP unexposedexposed ovariole.(G to H) CantonS transferase dUTP nick end labeling (TUNEL) staining performed on exposed fly ovaries.(I to J) HisGFPTUNEL staining.For (A) and (B), error bars represent regular error (n biological replicates from which ovaries had been scored for each and every group) (p ).Scale bars, m..eLife.The following figure supplement is out there for figure Figure supplement .Stagespecific apoptosis is induced following wasp exposure..eLife.Kacsoh et al.eLife ;e..eLife.ofResearch articleCell biology Neuroscienceat equivalent levels.Developmentally regulated cell death is ordinarily expected to do away with latestage nurse cells in maturing oocytes, thus, serving as an internal manage for the degree of detected apoptosis in exposed and unexposed females (Supplementary file A,B).Related for the reduced oviposition behavior observed, this physiologically triggered apoptosis E4CPG price particularly of stage egg chambers persisted well beyond the period of initial wasp exposure (Figure A,B, Supplementary file C,D).Flies continue to consume highprotein diet following wasp exposure but nevertheless depress ovipositionWe viewed as the possibility that exposure to wasps could change fly feeding behavior, and subsequent poor nutrition could trigger the midoogenesis checkpoint (DrummondBarbosa and Spradling,).We gave both exposed and unexposed flies a highprotein yeast food stained with PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/21487883 red meals dye to visualize meals intake.We identified that each waspexposed and unexposed flies exhibited a equivalent quantity of highprotein yeast food intake even when offered a choice to feed on typical food with no yeast by visualizing the red dye within the fly abdomens (Figure A , Figure figure supplement A).The red yeast paste was placed on immediate Drosophila media, which turns blue upon make contact with with water, allowing us to visualize no matter whether flies are preferring high (red) or low (blue) nutrient meals (Figure figure supplement E).We located that even within the presence of highprotein yeast meals, exposed flies nevertheless depressed oviposition when in comparison with unexposed controls, as well as having apoptosis induced in the egg chamber stage checkpoint (Figure E , Figure figure supplement M , Supplementary file E).Hence, the midoogenesis apoptosis checkpoint triggered in exposed flies just isn’t because of a poor nutrition intake.These da.