Worth was established S the S therapy, was amended using the digestate containing a high S-SO4 2- concentration (Table 5). five). ARS moderately correlated PHOS (r = 0.58) which a higher S-SO42- concentration (Table ARS moderately correlated toto PHOS (r =0.58) which was statistically the highest in the remedy and lowest in the BC (Figure 2c). The final was statistically the highest in the S S therapy and lowest inside the BC (Figure 2c). The final determined enzyme was in comparison towards the the manage substantially improved in determined enzyme UREURE was in comparison tocontrol significantly enhanced in sulsulphur amended therapies + S and S (Figure 2d). phur amended treatments BCBC + S and S (Figure 2d).Figure 2. Soil activities of – glucosidase–GLU (a), arylsulfatase–ARS (b), phosphatase–PHOS (c),(c), and urease–URE Figure 2. Soil activities of – glucosidase–GLU (a), arylsulfatase–ARS (b), phosphatase–PHOS and urease–URE (d); (d); tested treatment options: BC–biochar, S–sulphur, + S–biochar and and sulphur. Imply SD. The distinctive letters express tested treatments: BC–biochar, S–sulphur, BC BC + S–biochar sulphur. Mean SD. The diverse letters express the the results of ANOVA Tukey’s HSD Posthoc Test–the statistical variations at significance level0.05.0.05. results of ANOVA Tukey’s HSD Posthoc Test–the statistical variations at significance level p pThe values of BR inside the BC and S S remedies had been significantly decrease comparedthe The values of BR inside the BC and therapies have been drastically reduced in comparison to towards the control (Figure 3a), displaying that aerobic decomposition is apparently TPX-0131 Autophagy negatively afcontrol (Figure 3a), showing that aerobic decomposition is apparently negatively impacted fected by the amendment respective enriched digestates. The co-enrichment of digestate by the amendment with the with the respective enriched digestates. The co-enrichment of digestate with each the biochar and elemental sulphur mitigates the negative of each and every from the with each the biochar and elemental sulphur mitigates the adverse impact effect of every single with the components on the the inside the soil. components around the BR in BR soil. As all SIRs correlated extremely or moderately positively with every single other, the differences all SIRs correlated extremely or moderately positively with every single other, the differences inside the respiration properties had been equivalent (Figure 3b ). For example, the BC and S treatrespiration properties have been related (Figure 3b ). By way of example, the BC and S treatments’ values were drastically reduce than the handle. In contrast, the BC + S digestate ments’ values considerably elevated or did not modify all SIRs and we assumed that the combined enrichment of improved digestate by biochar and sulphur mitigated the adverse impact of either BC or elemental Son by biochar and sulphur mitigated the adverse effect of either BC or elemental soil soil aerobes. Furthermore, the PCA (Figure A2) showed a optimistic connection amongst Son aerobes. Moreover, the PCA biplotbiplot (Figure A2) showed a good connection all kinds of soil of soil respiration except for Glc-SIR. amongst all typesrespiration except for Glc-SIR.Agronomy 2021, 11, 2041 Agronomy 2021, 11, x FOR PEER REVIEW8 of 14 8 ofFigure 3. Basal respiration YB-0158 Purity trehalose SIR–Tre-SIR (b), (b), L-lysine SIR–Lys-SIR (c), L-alanine Figure three. Basal respiration (a),(a), trehalose SIR–Tre-SIR L-lysine SIR–Lys-SIR (c), L-alanine SIR– SIR–Ala-SIR (d), D-glucose SIR–Glc-SIR (e) and N-acetyl–D-glucosamine SIR.