F axis, whereas whereas an asterisk more than the whisker of a group indicates a important difference of that group are Figure two. ConGoralatide Autophagy centrations of per liter ( oles/L) and plotted on the ideal axis.to low concentraexpressed in micromoles total proteins and albumin in serum of with the manage group based on pigs exposed The plot illustrates Dunn’s post-test.the tions of ZEN (ZENlow; n = 10), high doses of ZEN (ZENhigh; n = 10), and manage (n = ten). Total median (line within box); 25th and 75th percentiles (box); 1.five instances the inter-quartile distance proteins are expressed in grams per ). Asterisks more than on the left axis, whereas of significance (Tukey; whiskers); and outliers ( ,liter (g/L) and plotted a bar indicate the levelalbumin values of are expressed in micromoles per liter ( oles/L) whisker of a the right axis. The plot illustrates Kruskal allis test, whereas an asterisk over the and plotted ongroup indicates a important differthe that group inside box); 25th and 75th percentiles (box); post-test. ence ofmedian (linewith the handle group according to Dunn’s1.5 occasions the inter-quartile distance (Tukey; whiskers); and outliers ( , ). Asterisks more than a bar indicate the level of significance ofKruskal allis test, whereas an asterisk more than the whisker of a group indicates a significant differ-g/L2.1.two. Lipid MetabolismToxins 2021, 13,Exposure to ZEN had a considerable effect on biochemical SB 271046 custom synthesis parameters associated with of 16 four lipid metabolism (Figure 3). At day 7, considerable differences in low-density (LDL), highdensity lipoprotein (HDL), and cholesterol (CHO) levels (p = 0.0032, 0.003, and 0.0033, 2.1.2. Lipid Concentrations of all 3 parameters were higher in respectively) had been observed. Metabolism Exposure to ZEN had ZENlow group in comparison with controls plus a substantial impact on biochemical parameters related signifiZENhigh pigs, but differences have been not with lipid metabolism (Figure 3). At day 7, substantial variations in low-density (LDL), high-density cant based on Dunn’s post-test. No cholesterol (CHO) levels (p = 0.0032, 0.003, and within the respectively) lipoprotein (HDL), and considerable variations were identified 0.0033, levels of totally free fatty acids (FFA) were observed. Concentrations of all 3 parameters were larger in ZENlow conat this time-point. Slightly reduced levels of HDL, LDL, and CHO group compared to controls and ZENhigh pigs, but variations were not substantial according centrations have been observed in pigs exposed to ZEN in comparison to controls at day 21, altto Dunn’s post-test. No considerable differences have been identified in the levels of cost-free fatty acids hough differences have been not important, except for the ZENhigh group compared to manage (FFA) at this time-point. Slightly reduced levels of HDL, LDL, and CHO concentrations were observed turn, drastically lower levels of day 21, though differences on for HDL (p-value 0.05). Inin pigs exposed to ZEN when compared with controls at FFA have been quantified had been not important, except for 0.001). Dunn’s post-test was for HDL (p-value FFA animals exposed for 21 days to ZEN (p the ZENhigh group compared to controlsignificant for 0.05). In turn, considerably lower levels of FFA had been quantified on animals exposed for 21 days concentrations in ZENlow and ZENhigh groups when compared with controlconcentrations in ZENlow and to ZEN (p 0.001). Dunn’s post-test was important for FFA (p 0.01 and p 0.001, ZENhigh groups when compared with handle (p 0.01 and p 0.001, respectively). respectively).Fig.