N (SON), [-0.1367, 0.4365] [-0.2072, 0.3661] [-0.6312, -0.0579] [-0.2176, 0.3557] [-0.405, 0.1683] [-0.3396, 0.2337] [-0.3321, 0.2412] [-
N (SON), [-0.1367, 0.4365] [-0.2072, 0.3661] [-0.6312, -0.0579] [-0.2176, 0.3557] [-0.405, 0.1683] [-0.3396, 0.2337] [-0.3321, 0.2412] [-0.525, 0.0483] [-0.0905, 0.4828] [-0.4877, 0.0856]Big Data Cogn. Comput. 2021, 5,15 ofFigure A1. Three regions utilized to predict the effect of LC alterations on surface temperature: northern (green), central (yellow), and southern (red) Europe.
bioengineeringArticleCharacterization, Cytotoxicity and Anti-Inflammatory Impact Evaluation of Nanocapsules Containing NicotineCarolina Landau Albrecht 1,2, , Laura Elena Sperling 1, , Daikelly Iglesias UCB-5307 web Braghirolli 1 and Patricia Pranke 1,two,2Hematology and Stem Cell Laboratory, Faculty of Pharmacy, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 90.610-000, RS, Brazil; [email protected] (D.I.B.); [email protected] (P.P.) Postgraduate System in Physiology, UFRGS, Porto Alegre 90.610-000, RS, Brazil Stem Cell Investigation Institute (Instituto de Pesquisa com C ulas-Tronco), Porto Alegre 90.610-000, RS, Brazil Correspondence: [email protected] (C.A.L.); laurasperling@BI-0115 Inhibitor unisinos.br (L.E.S.)Citation: Landau Albrecht, C.; Sperling, L.E.; Iglesias Braghirolli, D.; Pranke, P. Characterization, Cytotoxicity and Anti-Inflammatory Effect Evaluation of Nanocapsules Containing Nicotine. Bioengineering 2021, 8, 172. https://doi.org/ 10.3390/bioengineeringAbstract: (1) Background: Nanotechnology is an emerging field that may be applied inside the biomedical location. In this study, Eudragit nanocapsules (NCs) containing nicotine were produced. Nicotine would be the main alkaloid found in tobacco and has anti-inflammatory properties. NCs containing nicotine could be employed as an adjuvant therapy in the treatment of inflammation inside the central nervous technique. (2) Strategies: Nanocapsules have been prepared by the interfacial deposition on the pre-formed polymer technique and characterized with regards to zeta potential, diameter, polydispersity index, pH, encapsulation efficiency (EE), stability and sustained release profile. In vitro tests with all the PC12 cell line were performed, like MTT, LIVE/DEAD and ELISA assays, to verify their cytotoxic and anti-inflammatory effects. (three) Results: The nanocapsules presented satisfactory values on the characterization parameters; nevertheless, poor encapsulation was obtained for nicotine (eight.17 0.47). The in vitro tests showed that the therapy with nanocapsules decreased cell viability, which suggests that the Eudragit or the volume of polymer on top in the cells could possibly be detrimental to them, because the cells have been able to survive when treated with bulk nicotine. ELISA showed an increment in the expression of IL-6 and IL-1, corroborating the hypothesis that NCs have been toxic for the cells because of the boost inside the levels of those pro-inflammatory cytokines. (four) Conclusions: This study demonstrates that NCs of Eudragit present toxicity. It really is consequently necessary to enhance NC formulation to acquire better values for the encapsulation efficiency and decrease toxicity of these nanodevices. Search phrases: nanocapsules; Eudragit; nicotine; inflammation; PCAcademic Editor: Ilaria Fratoddi Received: 30 September 2021 Accepted: 27 October 2021 Published: 3 November1. Introduction Nanotechnology is an emerging interdisciplinary field that combines chemistry, physics, engineering and biology and has been extensively employed in recent years within the health analysis area. It aims to make functional components by controlling the matter within the nanometer scale, thus, generating supplies with ne.