He experiment plus the extract was administered as single dose and
He experiment and the extract was administered as single dose and observed for the mortality up to 48 h study period (quick term toxicity). Determined by the quick term toxicity profile, the next dose of the extract was determined as per OECD recommendations No.420. The maximum dose tested (2000 mg/kg) for LD50. In the LD50, doses like 1/20th, 1/10th and 1/5th were chosen and considered as low, medium and higher dose i.e., one hundred mg/kg, 200 mg/kg, 400 mg/kg respectively to carry out this study.Experimental DesignThe diuretic activity of alcoholic extract of roots of Cissampelos pareira in albino rats was studied by the Lipschitz Test [16-18]. Male Albino rats were divided into 5 groups of 6 rats in every. The group I serves as typical control received vehicle (CMC 2 in standard saline 10 ml/kg b.wt), the group II received Furosemide (10 mg/kg, p.o) in car; other groups III, IV, V had been treated with low, medium, and higher doses of alcoholic extract of roots of Cissampelos pareira in car and straight away after the extract remedy each of the rats had been hydrated with saline (15 ml/kg) and placed within the metabolic cages (two per cage), specially developed to separate urine and faeces andS. no. 1 2 three four 5 groups Manage (10 ml/Kg b. wt) Standard (Frusemide ten mg/kg b.wt) Alcoholic extract of roots of C.pareira Low (one hundred mg/kg b.wt) Alcoholic extract of roots of C.pareira Medium (200 mg/kg b.wt) Alcoholic extract of roots of C.pareira Higher (400 mg/kg b.wt)DISCUSSIONMedicinal plants and botanicals offer you a natural safeguard against illnesses and are a substantial treatment for certain ailments. Diuretics have proved to be really useful inside the remedy of mild to moderate hypertension and also in enhancing the impact of other antihypertensive agents. Diuretics relieve pulmonary congestion and peripheral oedema. These agents are useful in minimizing volume more than load and relieve orthopnea and paroxysmal nocturnal HDAC6 custom synthesis dyspnoea [19] in CCF and acute left ventricular failure. They decrease plasma volume and subsequently venous return towards the heart. This decreases the cardiac perform load, oxygen demand and plasma volume and also decreases blood stress. Thusna+ mmol/l 113.03 + two.16 191.05+2.09 129.40+2.*** ***total urine Vol (ml/kg b.wt/5 h) 13.45.02 22.23.01 15.20.*** ***K+ mmol/l 51.09 + 1.51 87.81+1.60 64.13+1.*** ***Cl- mmol/l 82.95 + 1.42 129.06+1.67*** 94.42 + 1.73*** 109.44+1.20*** 121.39+2.00***17.41.02*** 20.46.***164.99+2.00*** 184.53+2.***77.93+2.67*** 85.11+1.***[Table/Fig-1]: Effect of alcoholic extract of roots of Cissampelos pareira on urine volume and electrolyte concentration in hydrated rat model in albino rats Values expressed as mean S.E.M.,n=6, Significance at p0.05*, p0.01**, p0.001***, Compared with handle group (One particular Way ANOVA followed by Dunnetts `t’ test).Journal of Clinical and Diagnostic Analysis. 2014 May perhaps, Vol-8(5): HC01-HCjcdr.netSuresh Babu Sayana et al., Evaluation of Diuretic Activity of Alcoholic Extract of Roots of Cissampelos Pareira in Albino Ratssaponins, organic acids [1,17], steroids, carbohydrates, tannins, phenolic compounds, terpenoids [22], alkaloids [23], glycosides [24], sterols [25], sesquiterpenes aminoacids, carotinoids [26] in various plant extracts. Alcoholic extract of roots of Cissampelos pareira was identified with most of these plant ATR site phytochemical substances pointed out above. Therefore it might be reported that the observed diuretic activity is resulting from these above phytoconstituents.CONCLUSIONResults showed that single dos.