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J Ginseng Res 39 (2015) 221eContents lists out there at ScienceDirectJournal of Ginseng Researchjournal homepage: ginsengres.orgResearch articlePreparation of minor ginsenosides C-Mc, C-Y, F2, and C-K from American ginseng PPD-ginsenoside working with special ginsenosidase type-I from Aspergillus niger g.Chun-Ying Liu 1, 2, Rui-Xin Zhou 1, Chang-Kai Sun 3, Ying-Hua Jin 2, Hong-Shan Yu 1, , Tian-Yang Zhang 1, Long-Quan Xu 1, Feng-Xie Jin 1, College of Biotechnology, Dalian Polytechnic University, Dalian, People’s Republic of China Crucial Laboratory for Molecular Enzymology and Engineering of your Ministry of Education, College of Life Science, Jilin University, Changchun, People’s Republic of China three Institute for Brain Problems, Dalian Medical University, Dalian, People’s Republic of China2a r t i c l e i n f oArticle history: Received 14 October 2014 Received in Revised form 11 December 2014 Accepted 19 December 2014 Offered online 31 December 2014 Keywords: Aspergillus niger g.848 Panax quinquefolius ginsenosidase type-I minor ginsenosides protopanaxadiol-type ginsenosidesa b s t r a c tBackground: Minor ginsenosides, those getting low content in ginseng, have higher pharmacological activities. To receive minor ginsenosides, the biotransformation of American ginseng protopanaxadiol (PPD)-ginsenoside w.