CD4+ and CD8+ T cells were isolated from PB samples using anti-CD4 or anti-CD8 microbeads (Myltenyi Biotec, Bergisch Gladbach, Germany) following manufacturer’s protocol
Mononuclear cells (MNC) have been isolated by Ficoll-Hypaque (Sigma) density gradient.CD4+ and CD8+ T cells were isolated from PB samples using anti-CD4 or anti-CD8 microbeads (Myltenyi Biotec, Bergisch Gladbach, Germany)…