The amplicon was then probed with two primers, an upstream allele certain primer with a special 24 nucleotide FlexMAPTMTAG sequence extension (LuminexH Corporation, Austin, TX) and a downstream 59 phosphorylated/39 biotinylated conserved sequence primer
An estimated 600,000 little ones per year are infected with HIV-1, the bulk of them by mother-to-baby transmission (MTCT) . Most of these children stay in sub-Saharan Africa, exactly where…