The helicase domain of Mov10 is not essential for HIV-1 restriction. Virions have been produced by cotransfection of 293T cells with HIV-one vector and possibly empty vector or Mov10, Mov10 N-terminus, Mov10 C-terminus or the putative helicase motif mutant expression plasmids
In experiments wherever Mov10 was also cotransfected, the plasmid was applied in quantities ranging from .5 mg to .002 mg, which corresponds to ratios of HIV-1/Mov10 of one/6 to 1/1500.…