The expression of pluripotency markers this kind of as Oct-three/4A, Klf-four, c-Myc and Sox2 was evaluated at the mRNA level by real-time RT-PCR (Fig 2A) to evaluate whether or not MCPIP1 may be associated in regulation of stem cell-connected genes

To evaluate the impact of MCPIP1 on capabilities of BM-derived MSCs, cells have been transduced with retroviral vectors carrying the sequence coding MCPIP1 (MCPIP1-MSCs). Empty vectortreated (Puro) or untreated (Handle)…